It is possible to resume an acquisition that stopped for whatever reason. The following steps can be performed even if MATLAB crashed or the acquisition was interrupted by a power cut.
Perform the following steps to resume an acquisition:
Select Sample > Resume Acquisition from the BakingTray menu. Load the recipe file from the acquisition directory containing the sample you wish to resume.
You will be asked to confirm whether you wish to resume the acquisition.
Press "Yes" and double-check the parameters in the recipe and prepare GUI.
You will then be presented with a new GUI asking how the acquisition should be resumed. e.g. if the last section was partially completed, should the existing data be discarded and the section re-imaged.
You are responsible for turning the PMTs back on. Similarly for ensuring the laser is on and the shutter is open.
The Acquisition GUI will now appear. Press "Bake" to start the acquisition.
It is not necessary to do another preview scan. If you are re-starting the acquisition because of an issue related with the auto-ROI, you should do another preview and another Auto-Thresh before pressing Bake.
Each resumed acquisition will create a new recipe file in the acquisition directory. This is OK and will not lead to any problems.