Any sample that can be embedded in agar, cut on a vibratome, and contains a fluorescent signal can be imaged. Fixed tissue exhibits rich autofluorescence so label-free structural imaging is possible. Imaging of a wide range of fluorophores is, of course, also possible. The sample should ideally be brightly labeled to allow for short integration times and therefore faster imaging. Typical use cases that work well include counting labeled somata, mapping dye-labeled electrode tracks in brains, tracing bulk fibre projections in brains. Higher resolution scenarios, such as brain-wide tracing of individual axons is possible but very challenging.